Categories
Biology

I am attaching a file titled ” full details” I roughly wrote everything about my

I am attaching a file titled ” full details” I roughly wrote everything about myself and my journey and also within the same file I also listed what universities want in their personal statement. please eliminate any background noise and unwanted stuff that doesn’t fit in the story and make it interesting to read.

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Biology

A 25-year-old male was referred for further testing following a diagnosis of kid

A 25-year-old male was referred for further testing following a diagnosis of kidney disease
and hypertension. The results indicated that he had primary Antiphospholipid syndrome
(APS). Can you discuss the pathophysiology of the disease and discuss the diagnostic
laboratory assays used to confirm the disease. Provide an algorithm to show how you would
reach the differential diagnosis

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Biology

AbstractIntroduction: must include: the given experimental questions and the gen

AbstractIntroduction: must include:
the given experimental questions and the generated null & alternative hypotheses clearly and correctly stated. Convey to the reader the purpose of experiment & include a brief summary of known facts and background information (with citations from peer reviewed sources)MethodsResults: must include:- boxplots of bacterial colony growth for each growth media type (plate type). All groups’ plots must be reported on the same graph!
– a single table reporting each groups’ calculated transformation efficiency for ALL MEDIA TYPES where transformation occurred
– a series of appropriate t-tests addressing the experimental question “”How does growth media (plate type) affect the growth of bacterial colonies?”DiscussionConclusion

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Biology

Title Your title should be informative and concise Lay summary (10%) FUTURE TENS

Title
Your title should be informative and concise
Lay summary (10%) FUTURE TENSE
This section should provide a general overview of the background of your project. The purpose is to provide a general reader (i.e. someone without a scientific background, with the purpose of your project). You should try and make this as accessible as possible by avoiding complex scientific terminology or references to literature.Background (30%) PRESENT TENSE
This section should provide a general overview of the background of your project. The lay summary is for general readers while this section is for readers with a background in your subject area. Within the background should provide a clear, detailed overview of the literature in this area. This section should be fully referenced and should lead up to a clearly stated aim and hypothesis.- Cystic Fibrosis
– Main cause of morbidity and mortality – chronic infections etc
– lung microbiome differences in general / based on sex
– speak about current study ( see cuthbertson paper)
investigating lung infections between genders – what the dissertation will be aboutetc etc ….(every point should be asked with reasoning and referencing)Methods (30%)
This section should provide a clear, detailed and full account of the proposed methodology of your project. This should also include how any ethical and health and safety issues will be overcome. A plan of what statistical analysis you will perform on the data you will gather should be included at the end of the section.- retrospective study as study already completed (cuthbertson paper)
we are just going to describe how we will analyse data
– describe patient group and data – studying to look into…
– No patient identifiers – all been removed
– talk about approaches will be used to investigate it
– statistical analysis :
multi various statistical approaches
look at diversity – FISH kristkal analysis
composition different
– fishers indexetc etc ….Key outcomes and impact (15%)
This section should outline the key outcomes of your proposed research project i.e. what will be the main points of interest from this research project. In addition, you should highlight what impact the results of this project will have in terms of generating new intellectual property, products or services as well as the effect that your research may have to the general public.Milestones (10%)
This section should provide a timeline of when the main milestones of your research project will be completed.
GANT CHART TIMELINEReferencing and layout (5%)
Referencing should be included using the Harvard format. You should format the layout of your work/document to make the proposal easy to read and understand.———————————————————————————————————- what. is CF and CF lung infection
– lung microbiome in CF (see Cuthbertson paper)
– microbiome and sexhttps://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-020-00810-3

Categories
Biology

What is the first thing to do after putting a weighing boat on the balance?

Directions
Accurately measuring the volume of liquids, weighing chemicals, and adjusting the pH of
solutions are routine procedures in a working laboratory environment. This assignment is
designed to provide you with an overview of the general skills and knowledge you would need to
perform such tasks.
Before completing this assignment, you should ensure you have read your textbook –
particularly the section entitled pH, Buffers, Acids, and Bases. Answers should be concise and
well written. Make sure you correctly explain your thought process and provide all the necessary
information.
Question 1
The pH of a solution describes its acidity or alkalinity: Describe how pH and H3O
+
concentration
are related and explain why diluting an acid raises the pH, but diluting a base lowers the pH.
Question 2
Phosphate Buffered Saline (PBS) is a commonly used buffer for experiments in biology because
its pH and ion concentrations are similar to those in mammalian organisms. It works in a similar
fashion to the blood plasma buffer mentioned in the textbook, but using dihydrogen phosphate
ions and hydrogen phosphate ions for buffering through the following chemical reaction:
H2PO4

(aq) ⇆ H
+
(aq) + HPO4
2-
-(aq)
The equilibrium arrows depict that the phosphate ion (H2PO4-
) is dissociating further into two
component ions in solution, but at the same time H+ and HPO42-
ions are combining
simultaneously to form phosphate in solution. So, at any given point in time, and under the
appropriate conditions, there is an equal quantity of dissolved ions and combined ions in
solution. There is therefore always a hydrogen ion donor and an acceptor in solution.
Based on the equation above, which ion plays the role of hydrogen-ion donor (acid) and which
ion plays the role of hydrogen-ion acceptor (base) in PBS?
Question 3
The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the
protocol to make 1 litre of 10x concentrate PBS.
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Combine the following:
• 80g NaCl
• 2g KCl
• 14.4g Na2HPO4 (dibasic anhydrous)
• 2.4g KH2PO4 (monobasic anhydrous)
• 800mL distilled H2O
1. Adjust pH to 7.4 with HCl
2. Add H2O to 1L
3. Autoclave for 20 minutes on liquid cycle. Store at room temperature.
Which ions are being produced by this process, assuming that each of the chemical compounds
dissociate into their constituent parts once they are dissolved in
water?
Question 4
Preparation of the correct buffer is key to any good biological experiment and it is important
that you understand how to calculate the mass of each chemical required to make that buffer
and what the resulting concentration of those constituents will be in moles per litre.
Your text book explains that moles are just a way to express the amount of a substance, such
that one mole is equal to 6.02 x 1023 particles of that substance. These particles can be can be
atoms, molecules, ions etc, so 1 mole of water is equal to 6.02 x 1023 water molecules, or 1 mole
of Na+
is equal to 6.02 x 1023 Na+
ions. Since different chemicals have different molecular
weights (based on the number of protons and neutrons each atom contains) 1 mole or 6.02 x
1023 atoms of oxygen (O) will have a mass of 16g whereas 1 mole or 6.02 x 1023 atoms of sodium
(Na) will have a mass of 23g
If you need more information on moles, please read Encyclopedia Britannica’s Moles website.
Although you may sometimes see it written as g/litre, the concentration of solutions is more
often described in term of molarity since it better defines the chemical properties of a solution
because it is proportional to the number of molecules or ions in solution, irrespective of
molecular mass of its constituents. However, it is not possible to measure moles on a laboratory
balance, so in the first instance chemicals are measured by mass (milligrams, grams, kilograms
etc) and the number of moles is calculated using the known molecular mass (often called
molecular weight and abbreviated to M.W.) of the chemical. As indicated earlier, the molecular
mass of a chemical is based on the number of protons and neutrons that is contained in each
atom (eg NaCl is made up of one molecule of Na, M.W. = 22.99g and one molecule of Cl, M.W. =
35.45g, so the M.W. of NaCl is 58.44g). These values can be found in the periodic table however
the molecular mass of chemicals is generally provided by any vendors of the products and so can
also be found on various suppliers’ websites.
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When the concentrations of solutions are as described as ‘molar’, this refers to number of moles
per litre eg a 3-molar solution of NaCl will contain 3 moles of NaCl in 1 litre of water. As
indicated above, the M.W. of NaCl is 58.44g, so in 58.44g there are 6.02 x 1023 NaCl
molecules ie 1 mole. So, for 3 moles of NaCl you would need to dissolve 175.32g in 1 litre of
water (175.32/58.44 =3) whereas If you only dissolved 29.22g of NaCl in 1 litre of water this
would result in a 0.5 molar solution (29.22/58.44= 0.5)
1. As directed you need to check the periodic table and pick up the atomic masses for each
of the component atoms in the compounds. For example, for NaCl you need to pick the
atomic weight of both sodium and chlorine and then add them to two decimal places to
obtain the molecular mass of NaCl. Be sure to multiply the atomic masses by the number
of individual atoms of the same element present in each compound before finally adding
to the masses of other component atoms of other elements to make up the total
molecular masses.
2. From there you can calculate the number of ‘moles’ of each compound by multiplying
the provided weight of compound used in the PBS solution by their respective molar
mass conversion factors (i.e. 1L divided by the molecular mass you have calculated in the
first step)
3. Now, the molarity in Mol per Litre (mol/l) is given by the ‘number of moles’ of each
compound (calculated in step 2 above) divided by the given volume of the solution.
For more information on how to calculate morality, refer to wikiHow’s 4 Ways to Calculate
Molarity.
Using periodic table found in your textbook, calculate (to 2 decimal places) the molecular mass
for each of the compounds used to make PBS.
Create the following table and fill it in with the mass of each component required to make 1 litre
of 10 x PBS (the recipe for 10x PBS is below question 2) and their final molar concentration in the
buffer calculated as described above.
Compound formula Molecular
mass (in
g/mol)
Mass of
compound
per litre of
10x PBS (in
g)
Molar
concentration
(in mol/l)
NaCl
KCl
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Na2HPO4
KH2PO4
Question 5
As previously stated, the concentration of NaCl, KCl and Phosphate in working strength 1 x PBS is
0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4 How do they compare to the
concentrations you calculated for 10x PBS?
Watch the following videos and answer the remaining questions
• “Using an Electronic Balance” from Bio-Rad tutorials
• “Using a pH Meter” from Bio-Rad tutorials
• “Making a PBS solution” from Community College Consortium for Bioscience Credentials
Question 6
What is the first thing to do after putting a weighing boat on the balance?
Question 7
If you have excess reagent on the weighing boat, what should you avoid doing and why?
Question 8
If you had the choice between a 1-litre beaker and a 1 litre graduated cylinder, which one should
you use to measure volumes with maximal precision when making 1 litre of PBS? (you can
perform an internet search to find this if you are not sure of the answer)
Question 9
What should be done before measuring an unknown pH of a solution using a pH meter?
Question 10
The recipe for PBS says to dissolve compounds in 800 ml of water, adjust the pH to 7.4, then add
water up to 1 litre. The final pH should still be 7.4, because the pH of buffer solutions remains
stable when they are diluted as long as the concentration of its constitutive acid and base is not
too low.
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Why do you think the protocol does not say to dissolve compounds directly in 1 litre of water?
Question 11
The PBS protocol above says to adjust pH to 7.4 with HCl. What does this imply on the pH of 10x
PBS before adjusting the pH, would it be greater or smaller than 7.4?
Question 12
The last step in the protocol is to autoclave the 10x PBS solution. Why do you think this step is
important? Look up the definition of autoclave if you are unsure what it means.
Question 13
Taking into account your response to question 5, now that you have made a 10x PBS solution,
describe how you would prepare 1 litre of 1x working solution PBS, including which glassware
you would use. Will you need to adjust the pH again?

Categories
Biology

This is a lab workbook complete each section I will upload the results from each

This is a lab workbook complete each section I will upload the results from each sections. For the second practical include 5 separate graphs, read through the questions it will show you which graphs to complete. For the 3rd practical (which is part completed) great two graphs one for the examples (Hand drawn) the second is on excel. I will keep you updated with the results and what types of graphs need doing. I haven’t uploaded the results for the technique 4 and 5
1.5 spaces

Categories
Biology

This is the revised writing assignment where you just follow up the feedback giv

This is the revised writing assignment where you just follow up the feedback given and make the revision. I have uploaded the assignment that was submitted and screenshots of the feedback. Please follow the feedback carefully. Also, I have uploaded the instructions for the initial assignment and the revised assignment instructions. Finally, I have uploaded the article for this assignment. Please follow the instructions and feedback carefully. One last feedback is:I think you have a good idea of what is happening in the paper but were a bit short on the details here. Try to go back and give as much detail as you can about the experiments you are summarizing, the questions you want to answer, etc. Make sure you answer all of the points and guidelines.

Categories
Biology

This assignment is a set of 6 questions with a word limit of 2600 for the entire assignment.

This assignment is a set of 6 questions with a word limit of 2600 for the entire assignment. Half of the questions require shorter answers than others. This will require doing some article searches and reading. Please include at least 3-4 references per question (scientific articles are the preferred source). Please be specific about the mechanism involved in various biological processes, read the question carefully and answer thoroughly.

Categories
Biology

What the results mean.

I want a summary of this article but must be include these 3 components which are introduction, body and discussion!
Introduction is the background information before the study which is half is in the abstract and the rest is in the 2 paragraphs.Body paragraphs are each figure which are 4 figures so it should be 4 different body paragraphs. In this format for each body paragraph, start off with the technique being used. Next, put the results of the experiments. Finally, you end each body paragraph off with a conclusion of what was concluded by the results.Discussion contains 2 parts. The first half is the summary of the results. what the results mean. The second half of the discussion is what was concluded by the paper. The discussion if you read the paper comes from the very last paragraph of the paper!
Please no quoting from the article at all and follow the rubric
Thanks ?? much appreciated

Categories
Biology

Https://www.distancelearningcentre.com/access_2020/assessments/biology/prac_inv_

https://www.distancelearningcentre.com/access_2020/assessments/Biology/Prac_Inv_TAQs.pdf
Password
X8Gf7BbPWPlease read the each TAQ & write assignment accordingly.
Please pay attention to the word count for each TAQ.
I will upload an answer sheet seperately for you to upload all answers for each TAQ onto.
(Please msg if you do not understand)
For full assignment wors count is 2500 but each TAQ has a certain amount of words
Answer sheet is attached below.
PLEASE DO NOT COPY PREVIOUS WORK AS IT GOES THROUGH TURN IT IN
PLEASE SEND A DRAFT COPY
Any questions do not hesitate to ask
Deadline is 25/11